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A second method for induction of a transmissible entity in the control of cancer in mice
Abstract:
Spontaneous tumour-bearing mice of the C3H/St and subline C3HB/St inbred strains received injections of three nucleosides, adenosine, 6-methyl adenosine, and 5-methyl cytidine, prepared in the same molecular concentration as they had occurred in an alcohol-soluble liver extract which in previous work had suppressed tumour growth. Two transmissible entities appear to be present and they show different degrees of effect upon the growth and regression of spontaneous tumours of mammary gland origin (adenocarcinoma). The first transmissible entity (TE) occurred in the lineal descent of mice following injection of the liver extract. The second entity (TE2) appeared following injection of 5-methyl cytidine. TE manifested its maximum effect in suppressing cancer in mice during the 20th generation of a lineal descent following the injection of the original liver extract into a mother of the cancer proband. TE2 appears to be optimal in controlling cancer in the F11 generation of a second lineal descent derived from an original cancer proband of the C3H/St inbred strain injected with 5-methyl cytidine.
Insights
Researchers investigated nucleoside injections in mice with spontaneous mammary tumors. Specific nucleosides, adenosine, 6-methyl adenosine, and 5-methyl cytidine, showed potential in suppressing tumor growth across generations.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Spontaneous mammary tumors in C3H/St and C3HB/St mice were studied.
- Previous research indicated an alcohol-soluble liver extract suppressed tumor growth.
Purpose of the Study:
- To investigate the effects of specific nucleosides (adenosine, 6-methyl adenosine, 5-methyl cytidine) on spontaneous mammary tumor growth and regression.
- To identify and characterize transmissible entities (TEs) responsible for tumor suppression.
Main Methods:
- Mice with spontaneous tumors received injections of adenosine, 6-methyl adenosine, and 5-methyl cytidine at concentrations found in a previously effective liver extract.
- Generational effects of these nucleoside injections on tumor development were monitored.
Main Results:
- Two distinct transmissible entities (TEs) were identified, influencing tumor growth and regression differently.
- The first transmissible entity (TE) showed maximum tumor suppression in the 20th generation following liver extract injection.
- The second transmissible entity (TE2), derived from 5-methyl cytidine, was optimal in controlling cancer in the F11 generation.
Conclusions:
- Specific nucleosides can induce transmissible entities that modulate spontaneous mammary tumor progression in mice.
- The timing of these entities' effects varies, with TE and TE2 showing optimal impact in different generations.