[The effect of cyclosporine on human endothelial cells in culture]

Cyclosporine A is a selective immunosuppressive agent, which significantly minimises graft versus host disease and graft rejection at transplanted patients. Its pharmacological function is many-sided but it is still the subject of research. The author researched direct acting of Cyclosporine A in the culture of endothelial cells. Homogeneous continuous line of endothelial cells from umbilical line had been exposed to Cyclosporine A in the concentration of 2 mg/ml for seven days. Cyclosporine A inhibited replication and proliferation in the culture of endothelial cells. Some abnormal morphological changes were shown: formation of vesicles, nuclear changes and explicit destruction of the cells. By determination cells' vitality, it was discovered significantly smaller number of cells, in a comparison with control cultures (P < 0.01), while by using of monoclonal antibodies on vWF: Ag, a quantitative change in its expressiveness in relation to the control group was proved.