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Bacteriocin release protein triggers dimerization of outer membrane phospholipase A in vivo
N Dekker1, J Tommassen, H M Verheij
1Department of Enzymology and Protein Engineering, Center for Biomembranes and Lipid Enzymology, Utrecht University, 3584 CH Utrecht, The Netherlands. n.dekker@chem.uu.nl
Journal of Bacteriology
|May 13, 1999
Abstract:
Bacteriocin release protein is known to activate outer membrane phospholipase A (OMPLA), which results in the release of colicin from Escherichia coli. In vivo chemical cross-linking experiments revealed that the activation coincides with dimerization of OMPLA. Permeabilization of the cell envelope and dimerization were characterized by a lag time of 2 h.