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Updated: Aug 4, 2026

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
Published on: August 13, 2016
Microtubule-based motor function in mitosis
1Department of Molecular and Cell Biology, 311 LSA University of California, Berkeley, CA 94720-3200, USA. heald@socrates.berkeley.edu
Abstract:
Microtubule-based motors are essential both for the proper assembly of the mitotic spindle and for chromosome segregation. Mitotic motors in the yeast Saccharomyces cerevisiae exhibit either overlapping or opposing activities in order to achieve proper spindle function, whereas the analysis of motors using vertebrate cytoplasmic extracts has revealed less functional redundancy. In several systems, biochemical, genetic and two-hybrid approaches have been used both to identify associated nonmotor proteins and to address the molecular mechanisms behind kinetochore movements during chromosome alignment and segregation.
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