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[Effects of low-molecular-weight Rehmannia glutinosa polysaccharides on p53 gene expression]
1Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China.
Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica
|May 14, 1999
Abstract
Aim:
To study the effect of low-molecular-weight Rehmannia glutinosa polysaccharides (LRPS) on p53 gene expression.
Methods:
The level of p53 gene expression was determined by quantitative polymerase chain reaction and autoradiography.
Results:
The levels of p53 mRNA in Lewis lung cancer tissue were 0.46, 1.52, 1.48, and 1.60 respectively after saline, LRPS 20 and 40 mg.kg-1, and cyclophosphamide 10 mg.kg-1, which were the effective doses inhibiting the growth of tumor tissue cells in vivo. LRPS markedly increased p53 gene expression.
Conclusion:
LRPS effect on p53 gene expression is one of the mechanisms of antitumor activity.