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Updated: Aug 3, 2026

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
Published on: April 18, 2016
Human dendritic cells express a 95 kDa activation/differentiation antigen defined by CMRF-56
B D Hock1, D B Fearnley, A Boyce
1Haematology/Immunology Research Group, Christchurch Hospital, New Zealand.
Researchers identified CMRF-56, a new antigen marker for human dendritic cells (DC). This early activation antigen is expressed on DC and a subset of lymphocytes, aiding DC research.
Area of Science:
- Immunology
- Cell Biology
Background:
- Dendritic cells (DC) play crucial roles in immunity.
- Limited cell surface markers, such as CMRF-44 and CD83, are known for human DC.
- A need exists for additional markers to study DC function and differentiation.
Purpose of the Study:
- To identify and characterize a novel cell surface antigen recognized by the CMRF-56 monoclonal antibody (mAb).
- To determine the expression pattern and kinetics of the CMRF-56 antigen on human immune cells.
- To compare CMRF-56 with existing DC markers (CMRF-44, CD83).
Main Methods:
- Monoclonal antibody production and characterization (mAb CMRF-56).
- Immunophenotyping of human leukocytes and cultured cells using flow cytometry.
- Antigen modulation studies involving temperature-dependent incubation.
- Western blotting to determine the molecular weight of the antigen.
Main Results:
- CMRF-56 recognizes a novel 95 kDa activation antigen on human dendritic cells.
- The antigen is not expressed on circulating leukocytes but is upregulated on DC upon in vitro culture.
- CMRF-56 expression is detected on various DC subsets, including Langerhan's cells and monocyte-derived DC.
- CMRF-56 binding is reduced reversibly at 37°C, unlike the irreversible modulation of CMRF-44.
Conclusions:
- CMRF-56 is a valuable early activation antigen marker for human dendritic cells.
- Its distinct expression kinetics and modulation properties differentiate it from CMRF-44 and CD83.
- CMRF-56 offers a new tool for investigating human DC biology and function.
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