Related Experiment Video
Updated: Mar 30, 2026
08:07
Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Published on: September 6, 2017
10.7K
Questionable expression of unstable DQ heterodimer containing HLA-DQA1*01:07
H Miyadera1,2, L B Bungener3, S Kusano4
1Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Tissue Antigens
|November 12, 2015
Summary
Human leukocyte antigen (HLA) DQA1*01:07 shows questionable cell-surface expression, particularly with DQB1*05:03. This HLA DQ allele exhibits low expression with DQB1*06:04, leading to its renaming to DQA1*01:07Q.
Area of Science:
- Immunogenetics
- Molecular immunology
- Human leukocyte antigen (HLA) complex
Background:
- Human leukocyte antigen (HLA) DQA1*01:07 was identified as a blank specificity.
- This allele segregated with the HLA-DQB1*05:03 haplotype.
- The blank specificity may result from poor typing sera reactivity or disrupted DQ heterodimer assembly.
Purpose of the Study:
- To investigate the cell-surface expression of the DQA1*01:07 allele.
- To determine the impact of co-expression with different DQB1 alleles on DQA1*01:07 surface expression.
Main Methods:
- Co-expression of DQA1*01:07 with various DQB1*05 and DQB1*06 alleles in fibroblast cells.
- Detection of cell-surface DQ expression using flow cytometry or similar techniques.
Main Results:
- Cell-surface DQ expression was detectable when DQA1*01:07 was co-expressed with DQB1*06:04.
- Expression was undetectable with other DQB1*05 and DQB1*06 alleles, including DQB1*05:03.
- DQA1*01:07 demonstrated null or low expression in the DQA1*01:07-DQB1*05:03 haplotype.
Conclusions:
- DQA1*01:07 may function as a phenotypically null allele in the context of the DQB1*05:03 haplotype.
- Low-level expression of DQA1*01:07 is possible with certain DQB1*06 alleles, such as DQB1*06:04, when expressed in trans.
- The questionable expression led to the renaming of DQA1*01:07 to DQA1*01:07Q.
Related Concept Videos
01:49Multiple Allele Traits
38.8K
The Concept of Multiple Allelism
38.8K
02:32Exon Recombination
4.3K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
4.3K
01:28Stability of Conjugated Dienes
4.7K
Introduction
A comparison of the enthalpies of hydrogenation of dienes reveals that conjugated dienes release less heat on hydrogenation, rendering them more stable than their nonconjugated analogs.
A comparison of the enthalpies of hydrogenation of dienes reveals that conjugated dienes release less heat on hydrogenation, rendering them more stable than their nonconjugated analogs.
4.7K
02:17Microtubule Instability
6.4K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
6.4K
00:48¹H NMR of Labile Protons: Deuterium (²H) Substitution
1.5K
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
1.5K