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Rapid insulin sensitivity test (RIST).

W W Lautt¹, X Wang, P Sadri

¹Department of Pharmacology and Therapeutics, Faculty of Medicine, The University of Manitoba, Winnipeg, Canada.

Canadian Journal of Physiology and Pharmacology

|May 18, 1999

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A refined protocol for the rapid insulin sensitivity test (RIST) in rats is presented. This standardized procedure enhances the assessment of insulin action and allows for reproducible insulin dose-response curve establishment.

Area of Science:

Pharmacology
Physiology
Endocrinology

Background:

The rapid insulin sensitivity test (RIST) was previously introduced to evaluate in vivo insulin action.
Further experience necessitated the development of a standardized operating procedure for RIST in rats.

Purpose of the Study:

To describe the current recommended standard operating procedure for the rapid insulin sensitivity test (RIST) in rats.
To detail the manufacture and use of an arterial-venous shunt for improved sample collection and drug administration.

Main Methods:

The RIST procedure involves establishing a stable arterial glucose baseline to maintain euglycemia after a 5-min insulin infusion.
Glucose infusion rate required to maintain euglycemia defines the RIST index.
An arterial-venous shunt facilitates rapid arterial sampling and intravenous drug delivery.

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Main Results:

Insulin administration via a 5-min infusion is more effective than a 30-s bolus.
No significant difference in RIST index was observed between pork-beef and human insulin.
The RIST index demonstrated sensitivity and reproducibility, allowing for dose-response studies and assessment of factors like hepatic parasympathetic nerve activity.

Conclusions:

The described standard operating procedure provides a reliable method for conducting the RIST in rats.
The RIST is a sensitive and reproducible tool for assessing insulin action in vivo.
This protocol supports further research into insulin sensitivity and related physiological mechanisms.