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Determination of aminoglutethimide enantiomers as dansyl derivative in human plasma by HPLC with fluorescence
H Y Aboul-Enein1, S Ozkirimli, T I Apak
1Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Abstract:
Determination of aminoglutethimide enantiomers as a dansyl derivative in plasma by HPLC has been achieved using cellulose tris(3,5-dimethylphenyl carbamate) chiral stationary phase known as Chiralcel OD, and a mobile phase consisted of ethanol-cyclohexane-methanol (95:5:2 v/v/v). The limit of detection for each enantiomer of aminoglutethimide using fluorescence detector was 20 ng ml-1.
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