[Effect on glucose on levansucrase synthesis by Gluconobacter oxydans]

The effect of glucose on synthesis of levansucrase was studied with Gluconobacter oxydans L-1. Glucose added to the nutritional medium containing sucrose was found to supress its degradation by G. oxydans L-1. At the same time, the lag phase became shorter. Synthesis of levansucrase was inhibited in the nutritional medium containing glucose while the enzymatic activity increased proportionally to the amount of bacterial biomass in the medium containing sorbitol. Addition of glucose to the nutritional medium containing sorbitol prior to inoculation results in a decrease of the differential rate of levansucrase synthesis. The repressory effect of glucose on the enzyme synthesis manifested itself most distinctly at its addition to the culture in the period of bacterial multiplication with the maximum rate.