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Related Experiment Videos

Survey for CAG repeat polymorphisms in the human MAP-2 gene.

N Kalcheva1, H M Lachman, B Shafit-Zagardo

  • 1Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Psychiatric Genetics
|May 21, 1999
PubMed
Summary
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Microtubule-associated protein-2 (MAP-2) gene CAG repeats are stable. This study found no evidence of repeat expansion in individuals with neuropsychiatric disorders, suggesting MAP-2 CAG repeat length is conserved.

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Microtubule-associated protein-2 (MAP-2) expression is altered in various neurological conditions like Alzheimer's disease and schizophrenia.
  • MAP-2, a gene expressed in the central nervous system, contains trinucleotide repeats in its 5' untranslated region (5' UTR).

Purpose of the Study:

  • To investigate polymorphisms and potential expansion of CAG repeats in the MAP-2 gene.
  • To determine if MAP-2 CAG repeat variations are associated with neuropsychiatric illnesses.

Main Methods:

  • Polymerase Chain Reaction (PCR) amplification of MAP-2 exon 1 CAG repeats from genomic DNA.
  • Sequencing and Short Tandem Repeat Polymorphism (STRP) analysis of PCR products from controls and patients with neuropsychiatric disorders.

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Main Results:

  • Analysis of 86 individuals (31 controls, 35 chronic schizophrenics, 20 others) revealed a consistent size for the seven CAG repeats in the MAP-2 5' UTR.
  • No evidence of CAG repeat expansion was found in the studied patient groups.

Conclusions:

  • The seven-copy CAG repeat in the 5' UTR of the MAP-2 gene is highly conserved across the general population.
  • CAG repeat expansion in MAP-2 is not a significant factor in the studied neuropsychiatric conditions.