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Structure of an extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83
C Vanhaverbeke1, C Bosso, P Colin-Morel
1Centre de Recherches sur les Macromolécules Végétales, CNRS and Université Joseph Fourier, Grenoble, France.
Abstract:
The extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83 was found to be composed of D-glucose and D-galactose in a molar ratio of 2:3. The primary structure of the polysaccharide was shown by sugar analysis, methylation analysis, FABMS, partial acid hydrolysis and nuclear magnetic resonance (NMR) spectroscopy to consist of a pentasaccharide repeating unit having the following structure: -->3)-alpha-D-Glcp-(1-->2)-beta-D-Galf-(1-->6)-alpha-D-Galp-(1-->6 )-alpha-D -Glcp-(1-->3)-beta-D-Galf-(1-->
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