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Precise quantitation of human parvovirus B19 DNA in biological samples by PCR
F Gruber1, F G Falkner, F Dorner
1Baxter-IMMUNO, Biomedical Research Center, Orth/Donau, Austria.
Abstract:
An application of a quantitative PCR-based method was developed for the detection of human parvovirus B19 DNA. The procedure was characterised according to guidelines for the validation of analytical procedures. Furthermore, the reliability was demonstrated by the correct quantitation of samples of an international collaborative study. This application might be useful for studies focussed on removal and/or inactivation procedures of human parvovirus B19 as well as for general screening purposes of biological materials.