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Separation and estimation of short-chain fatty acids and organic acids by thermodetective liquid chromatography
[Hokkaido Igaku Zasshi] the Hokkaido Journal of Medical Science
|September 1, 1976
Abstract:
Short-chain fatty acids and organic acids in aqueous solution were separated quantitatively by using a liquid chromatography. JLC-2A, which posseses a thermodetector with ion-exchange resin. A peak height of thermogram showing a thermal difference caused by adsorption and desorption of substance was proportional to the concentration. Each sample was detectable at least between approximately 10(-5) and 2 X 10(-3) moles. The "inter-day" coefficient of variation obtained from two peak heights of acetic and n-butyric acids was 1.9 and 2.3%, respectively.