Related Experiment Videos
Separation and identification of corticosterone metabolites by liquid chromatography--electrospray ionization mass
I Miksík1, M Vylitová, J Pácha
1Institute of Physiology, Academy of Sciences of the Czech Republic, Prague. miksik@biomed.cas.cz
Abstract:
High-performance liquid chromatography coupled to atmospheric pressure ionization-electrospray ionization mass spectrometry (API-ESI-MS) was investigated for the analysis of corticosterone metabolites; their characterization was obtained by combining the separation on Zorbax Eclipse XDB C18 column (eluted with a methanol-water-acetic acid gradient) with identification using positive ion mode API-ESI-MS and selected ion analysis. The applicability of this method was verified by monitoring the activity of steroid converting enzymes (20beta-hydroxysteroid dehydrogenase and 11beta-hydroxysteroid dehydrogenase) in avian intestines.