Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Testing tumors for microsatellite instability.

A de la Chapelle1

  • 1Human Cancer Genetics Program, Comprehensive Cancer Center, Ohio State University, Columbus, USA. delachapelle-1@medctr.osu.edu

European Journal of Human Genetics : EJHG
|June 3, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

No evidence for microsatellite instability in acute myeloid leukemia.

Leukemia·2017
Same author

The mutational oncoprint of recurrent cytogenetic abnormalities in adult patients with de novo acute myeloid leukemia.

Leukemia·2017
Same author

Mutations in the CCND1 and CCND2 genes are frequent events in adult patients with t(8;21)(q22;q22) acute myeloid leukemia.

Leukemia·2016
Same author

Adult acute myeloid leukemia with trisomy 11 as the sole abnormality is characterized by the presence of five distinct gene mutations: MLL-PTD, DNMT3A, U2AF1, FLT3-ITD and IDH2.

Leukemia·2016
Same author

MicroRNA-related sequence variations in human cancers.

Human genetics·2013
Same author

Recurrent and founder mutations in the PMS2 gene.

Clinical genetics·2012
Same journal

Parent and professional experiences of a clinical trial of prenatal and postnatal stem cell therapy for severe osteogenesis imperfecta.

European journal of human genetics : EJHG·2026
Same journal

Scoping review and recommendations for development and delivery of education resources for reproductive genetic carrier screening.

European journal of human genetics : EJHG·2026
Same journal

Australian parents' perspectives on extended genomic screening: what information to return and when?

European journal of human genetics : EJHG·2026
Same journal

Perspectives on phenotype in genetic testing for early-onset atrial fibrillation.

European journal of human genetics : EJHG·2026
Same journal

Systematic analysis of homozygous autosomal copy number losses in exomes improves diagnostic yield and uncovers ultra-rare recessive disorders.

European journal of human genetics : EJHG·2026
Same journal

Decoding splicing variants in high-throughput sequencing: a functional validation approach integrating deep learning tools.

European journal of human genetics : EJHG·2026
See all related articles

Analyzing tumor DNA with the single marker BAT-26 can assess microsatellite instability. Normal tissue analysis is only needed if aberrant alleles appear, simplifying cancer screening.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Microsatellite instability (MSI) detection methods are currently heterogeneous.
  • A 5-marker panel for MSI assessment was recently proposed.
  • Standardized and simplified MSI detection is needed for clinical applications.

Purpose of the Study:

  • To review and highlight a simplified method for assessing microsatellite instability.
  • To emphasize the utility of the BAT-26 marker for MSI screening.
  • To propose an efficient approach for mismatch repair deficiency screening in cancers.

Main Methods:

  • Review of existing literature on microsatellite instability detection.
  • Focus on the analysis of tumor DNA using the single marker BAT-26.

Related Experiment Videos

  • Conditional analysis of normal tissue DNA only when tumor aberrations are detected.

    • Main Results:

    • Microsatellite instability can be effectively assessed using the single marker BAT-26.
    • Normal DNA analysis is only required when aberrant alleles are observed in tumor DNA.
    • This simplified approach provides sufficient data for many applications, including screening.

    Conclusions:

    • A single-marker approach using BAT-26 is a viable and efficient method for assessing MSI.
    • This simplified procedure is adequate for screening colorectal cancers for mismatch repair deficiency.
    • The proposed method offers a practical solution to the heterogeneity in current MSI detection techniques.