Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exogenous expression of p16INK4a is associated with decrease in telomerase activity.

H Sawa1, H Kamada, T A Ohshima

  • 1Department of Neurosurgery, Kyorin University, School of Medicine, Mitaka, Tokyo, Japan.

Journal of Neuro-Oncology
|June 9, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early detection of Niemann-pick disease type C with cataplexy and orexin levels: continuous observation with and without Miglustat.

Orphanet journal of rare diseases·2020
Same author

Dissipation-Based Quantum Sensing of Magnons with a Superconducting Qubit.

Physical review letters·2020
Same author

Breaking the trade-off between fast control and long lifetime of a superconducting qubit.

Nature communications·2020
Same author

Probing Few-Body Nuclear Dynamics via ^{3}H and ^{3}He (e,e^{'}p)pn Cross-Section Measurements.

Physical review letters·2020
Same author

Helicity-Changing Brillouin Light Scattering by Magnons in a Ferromagnetic Crystal.

Physical review letters·2019
Same author

Ephrin type-A receptor 2 on tumor-derived exosomes enhances angiogenesis through the activation of MAPK signaling.

Die Pharmazie·2019

Exogenous p16INK4a expression suppressed glioblastoma cell growth and altered cell morphology. This study reveals p16INK4a

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Glioblastoma is an aggressive brain tumor with limited treatment options.
  • Understanding the molecular mechanisms regulating glioblastoma cell proliferation is crucial for developing new therapies.

Purpose of the Study:

  • To investigate the effect of exogenous p16INK4a expression on the biological characteristics of glioblastoma cells.
  • To determine if p16INK4a can modulate glioblastoma cell growth, cell cycle, and telomerase activity.

Main Methods:

  • Gene transfection of human glioblastoma U87MG cells with pVgRXR and pIND plasmids containing the wild-type p16 gene.
  • Regulation of p16INK4a expression using the ecdysone analogue, muristerone A.
  • Assessment of cell growth, morphology, cell cycle progression (Ki-67), and telomerase activity.

Related Experiment Videos

Main Results:

  • Exogenous p16INK4a expression reduced glioblastoma cell growth capacity.
  • Observed morphological changes included increased cell size and flattening.
  • p16INK4a expression inhibited cell cycle entry and decreased telomerase activity.

Conclusions:

  • Exogenous p16INK4a expression has a significant inhibitory effect on glioblastoma cell biological characteristics.
  • p16INK4a acts as a suppressor of glioblastoma cell proliferation and may be a potential therapeutic target.