Related Experiment Video
Updated: Aug 8, 2026
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
Published on: July 6, 2009
Numerical dose-compensated in vitro fertilization inseminations yield high fertilization and pregnancy rates
S Benoff1, G W Cooper, T Paine
1Department of Obstetrics and Gynecology, North Shore University Hospital-New York University School of Medicne, Manhasset 11030, USA. sbenoff@nshs.edu
Objective:
To evaluate in cases with morphologically abnormal sperm whether fertilization and pregnancy rates are increased by normalizing the number of sperm inseminated and whether biomarkers can identify cases of reduced or failed fertilization.
Design:
Prospective studies of sperm morphology and function.
Setting:
University hospital assisted human reproduction program.
Patient(S):
Partners of 308 women undergoing IVF.
Intervention(S):
Motile sperm populations were assessed for sperm head morphology, for surface receptors for mannose and progesterone binding, and the ability to undergo a free mannose-induced acrosome reaction. Zinc in seminal plasma was determined by atomic absorption spectroscopy.
Main Outcome Measure(S):
Sperm morphology was associated with fertilization and clinical pregnancy rates. Biomarker analyses were correlated with fertilization rates using Kruskal-Wallis tests, chi2 tests, and Spearman rank order correlations.
Result(S):
Fertilization and pregnancy rates after numerical dose compensation inseminations were indistinguishable between men with differing percentages of normal sperm. Biomarker deficits were identified irrespective of sperm head morphology in 96% of cases of reduced or failed fertilization.
Conclusion(S):
Fertilization and pregnancy rates in cases of abnormal morphology are optimized by inseminating at least 25,000 sperm/mL with normal acrosomes. Reduced or failed fertilization can be predicted by testing for molecular deficits in mannose receptor expression and mannose-stimulated acrosome loss.
Related Concept Videos
01:38Fertilization
01:24In Vitro Fertilization
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...