Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure and function of human prepro-orexin gene.

T Sakurai1, T Moriguchi, K Furuya

  • 1Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan. stakeshi@md.tsukuba.ac.jp

The Journal of Biological Chemistry
|June 11, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cumulative incidence of vancomycin-resistant Enterococcus faecium detection by patient characteristics or possible exposures: prioritization of patients for active screening culture.

The Journal of hospital infection·2024
Same author

Interobserver Reliability on Intravoxel Incoherent Motion Imaging in Patients with Acute Ischemic Stroke.

AJNR. American journal of neuroradiology·2022
Same author

Anisakiasis: The risks of seafood consumption.

Nigerian journal of clinical practice·2018
Same author

Paradoxical localized exacerbation of psoriatic eruptions triggered by secukinumab.

Clinical and experimental dermatology·2018
Same author

High-temperature-required protein A2 as a predictive marker for response to chemotherapy and prognosis in patients with high-grade serous ovarian cancers.

British journal of cancer·2016
Same author

Dedifferentiated endometrioid adenocarcinoma of the uterus: a case report.

European journal of gynaecological oncology·2016

Researchers cloned the human prepro-orexin gene, crucial for orexin peptides involved in feeding behavior. A specific gene promoter fragment successfully directed gene expression in targeted neurons in mice.

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Orexin-A and -B are potent orexigenic peptides regulating feeding behavior.
  • These peptides are localized in lateral hypothalamic neurons, a key feeding control center.
  • Orexin peptides are derived from a common precursor peptide, prepro-orexin.

Purpose of the Study:

  • To clone the full-length human prepro-orexin gene and its corresponding cDNA.
  • To characterize the structure of the human prepro-orexin gene.
  • To identify and test a promoter region capable of directing gene expression in orexin-producing neurons.

Main Methods:

  • Cloning of the human prepro-orexin gene and cDNA.
  • Analysis of gene structure, including exons, introns, and untranslated regions.

Related Experiment Videos

  • Generation of transgenic mice using a 5'-upstream promoter region linked to the lacZ reporter gene.
  • Immunohistochemical analysis of lacZ expression in transgenic mouse brains using anti-orexin and anti-melanin-concentrating hormone antibodies.

    • Main Results:

    • The human prepro-orexin mRNA encodes a 131-residue precursor peptide.
    • The gene comprises two exons and one intron over 1432 base pairs.
    • A 3.2 kb 5'-upstream promoter fragment directed lacZ expression specifically to lateral hypothalamic neurons.
    • These neurons were confirmed to be orexin-containing, as indicated by positive staining with anti-orexin antibody.

    Conclusions:

    • The identified genomic fragment contains necessary elements for appropriate orexin gene expression.
    • This promoter fragment is valuable for targeted gene expression in orexin-containing neurons.
    • The developed transgenic mice model can be utilized to study orexin gene regulation mechanisms.