Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Somatic hypermutation and B-cell malignancies.

J O Spencer1, D K Dunn-Walters

  • 1Department of Histopathology, UMDS, London, U.K. j.spencer@umds.ac.uk

The Journal of Pathology
|June 12, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The ageing human B cell repertoire: a failure of selection?

Clinical and experimental immunology·2015
Same author

A novel chronic lymphocytic leukemia subset expressing mutated IGHV3-7-encoded rheumatoid factor B-cell receptors that are functionally proficient.

Leukemia·2012
Same author

Mathematical analysis of antigen selection in somatically mutated immunoglobulin genes associated with autoimmunity.

Lupus·2010
Same author

Effects of age on antibody affinity maturation.

Biochemical Society transactions·2003
Same author

Related IgA1 and IgG producing cells in blood and diseased mucosa in ulcerative colitis.

Gut·2002
Same author

Sequence analysis of light chain genes from human intestinal plasma cells demonstrates that lambda genes are almost all in-frame and highly mutated and most kappa genes are highly mutated when in-frame and minimally mutated when out-of-frame.

European journal of immunology·2000

Immunoglobulin (Ig) V gene hypermutation introduces beneficial mutations in B cells, enhancing antibody affinity. This mechanism is crucial for understanding B-cell malignancies and their origins.

Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • Follicle center response involves immunoglobulin gene hypermutation.
  • This process introduces single base changes into immunoglobulin V (IgV) genes.
  • B cells with mutated IgV genes are selected based on antibody affinity.

Purpose of the Study:

  • To examine the characteristics of the IgV gene hypermutation mechanism.
  • To explore the application of IgV gene mutation analysis in B-cell malignancies.
  • To investigate B-cell ancestry and microenvironmental influence in lymphomas.

Main Methods:

  • Analysis of immunoglobulin V region (IgV) gene mutations.
  • Study of B cells and B-cell lymphomas.
  • Examination of mutation patterns in normal B cells and various lymphomas.

Related Experiment Videos

Main Results:

  • Hypermutation introduces predominantly single base changes into IgV genes.
  • Selection of B cells with mutated IgV genes increases humoral response affinity.
  • Mutated IgV genes identified in Hodgkin's Reed-Sternberg cells, challenging previous hypotheses.

Conclusions:

  • IgV gene hypermutation is a key mechanism in humoral immunity.
  • Analysis of these mutations aids in understanding B-cell malignancies.
  • Unexpected findings in lymphomas highlight the complexity of B-cell development and disease.