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[Application of competitive immunoenzyme assay for analysis of group B fumonisins]
G P Kononenko1, A A Burkin, E B Zotova
1Russian Research Institute of Veterinary Sanitary, Hygiene, and Ecology, Russian Academy of Agricultural Sciences, Moscow, Russia.
Prikladnaia Biokhimiia I Mikrobiologiia
|June 16, 1999
Abstract:
A varriant of competitive ELISA, making it possible to detect fumonisin B1 (up to 0.4 ng/well) was developed, based on the use of specific polyclonal antibodies obtained by immunization of rabbits with a horseradish peroxidase conjugate of fumonisin B1. The minimum concentration of fumonisin B in water-acetonitrile extracts of corn grain, dry corn products, and feeds, detected by the ELISA version developed is 0.2 mg/kg.