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Identification and characterization of brt1, a gene down-regulated during B-regulated development in Schizophyllum
1Friedrich-Schiller-Universität, Biologisch-Pharmazeutische Fakultät, Institut für Mikrobiologie, Mikrobielle Phytopathologie, Beutenbergstrasse 11, D-07745 Jena, Germany.
Abstract:
To identify genes regulated by the B mating-type genes a differential RNA display was established for the homobasidiomycete Schizophyllum commune and a gene, brt1, was identified. The mRNA concerned is highly abundant in monokaryotic mycelia and is down-regulated in matings in which either the B genes alone or else both the A and B genes are different between the mates. This places the gene brt1 under the control of the pheromone response system of S. commune encoded by the B mating-type loci. Sequence analysis revealed similarity to a novel protein family, two members of which have been shown to inhibit translational re-initiation.