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Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

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Protein-O-mannosyltransferases in virulence and development.

K B Lengeler¹, D Tielker, J F Ernst

¹Institut für Mikrobiologie, Molekulare Mykologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1/26.12, 40225, Düsseldorf, Germany.

Cellular and Molecular Life Sciences : CMLS

|November 3, 2007

Summary

Protein-O-mannosyltransferases (Pmt proteins) add mannose to proteins, impacting cell walls, differentiation, and virulence in fungi, bacteria, and mammals. This review explores Pmt roles in development and disease.

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Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Protein-O-mannosyltransferases (Pmt proteins) catalyze O-mannosylation, a crucial post-translational modification.
This modification is conserved across diverse organisms, including fungi, mammals, insects, and bacteria.
O-mannosylation plays vital roles in cell wall integrity, differentiation, and virulence.

Purpose of the Study:

To review the diverse functions of Pmt proteins.
To highlight the involvement of Pmt proteins in cellular differentiation, development, and virulence.
To discuss the implications of O-mannosylation in human diseases and pathogen virulence.

Main Methods:

Literature review of studies on Protein-O-mannosyltransferases.
Analysis of the roles of O-mannosylation in various organisms.
Examination of Pmt protein involvement in disease pathogenesis.

Main Results:

O-mannosylation is essential for fungal cell wall structure, differentiation, and virulence.
Pmt proteins are critical for mammalian and insect development; deficiencies cause muscular dystrophy.
O-mannosylation influences the virulence of Candida albicans and Mycobacterium tuberculosis.

Conclusions:

Pmt proteins are key regulators of fundamental biological processes across species.
Dysregulation of O-mannosylation is linked to human diseases and pathogen virulence.
Further research into Pmt proteins could yield therapeutic strategies for infectious diseases and developmental disorders.