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Related Experiment Videos

First the CDKs, now the DDKs.

L H Johnston1, H Masai, A Sugino

  • 1Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK NW7 1AA. ljohnst@nimr.mrc.ac.uk

Trends in Cell Biology
|June 17, 1999
PubMed
Summary

The Dbf4p-Cdc7p kinase complex initiates DNA synthesis in yeast. Similar protein kinases are found across eukaryotes, suggesting this DNA replication control mechanism is conserved.

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Area of Science:

  • Molecular Biology
  • Cell Cycle Regulation
  • Eukaryotic DNA Replication

Background:

  • In budding yeast, the Dbf4p-Cdc7p complex is crucial for initiating DNA synthesis.
  • Cdc7p acts as the catalytic subunit, while Dbf4p functions as an activating, cyclin-like subunit.
  • Dbf4p also directs Cdc7p to replication origins, targeting proteins like Mcm proteins.

Purpose of the Study:

  • To investigate the conservation of the Dbf4p-Cdc7p kinase complex and its role in DNA replication initiation across different eukaryotic organisms.

Main Methods:

  • Comparative analysis of protein sequences and functional studies in model organisms (budding yeast, fission yeast, metazoans).

Main Results:

  • Homologues of Dbf4p and Cdc7p have been identified in fission yeast and metazoans.
  • These homologous proteins also exhibit Mcm protein phosphorylation activity.
  • Evidence suggests a conserved function for these protein kinases in DNA replication.

Conclusions:

  • The Dbf4p-Cdc7p kinase activity, essential for DNA replication initiation, appears to be conserved throughout eukaryotic evolution.
  • This conservation parallels that observed for cyclin-dependent kinases (CDKs).

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