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Updated: Jul 29, 2026

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
10:14

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Published on: December 17, 2013

Three-colour, one-tube flow cytometric method for enumeration of CD4+ and CD8+ lymphocytes

W C Janssen1, J J Hoffmann

  • 1Department of Clinical Laboratories, Catharina Hospital, Eindhoven, The Netherlands.

Annals of Clinical Biochemistry
|June 17, 1999
PubMed

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...