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Isolation of rat sacral dorsal commissural neurons
1Department of Anatomy, K K Leung Brain Research Center, Fourth Military Medical University, Xi-an, China. patchclp@mail.fmmu.edu.cn
Unlabelled:
To isolate rat sacral dorsal commissural neurons (SDCN).
Methods:
Using enzymatic and mechanical dissociation techniques to isolate the neurons and using nystatin perforated patch technique to evaluate their functional state.
Results:
The isolated neurons exhibited good responses to excitatory and inhibitory amino acids. The responses of SDCN to N-methyl-D-aspartate were markedly potentiated by substance P and trans-1-aminocyclopentane-1,3-dicarboxylate, whereas GABA responses were significantly potentiated by diazepam, pregnenolone, and pentobarbital.
Conclusion:
This preparation provides a satisfactory model for exploring the mechanisms of the SDCN in nociception and antinociception.