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Pharmacokinetics of recombinant human granulocyte macrophage colony-stimulating factor in Macaca mulatta
1Department of Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
Aim:
To examine the pharmacokinetics of i.v. and s.c. recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) in Macaca mulatta.
Methods:
Plasma levels of rhGM-CSF were detected with sandwich enzyme-linked immunosorbent assay.
Results:
Plasma concentration-time curves after i.v. rhGM-CSF in monkeys were best fitted with 3-compartment model. The 1st, 2nd, and 3rd phase T1/2 were 0.05-0.07, 0.14-0.58, and 1.4-4.1 h. Cl and K10 were similar between different doses, respectively. Cmax was 0.93 +/- 0.16 microgram.L-1, Tmax was 2.65 +/- 0.14 h, and elimination T1/2 was 2.5 +/- 0.3 h after s.c. rhGM-CSF. The bioavailability after s.c. rhGM-CSF was 0.61.
Conclusion:
Pharmacokinetics of rhGM-CSF in Macaca mulatta provided a useful index for clinical trial.