Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Decrease of cAMP and increase of amino acids contents in mouse brain after dihydroetorphine tolerance].

M H Chen1, Q S Li, Y Shen

  • 1Department of Neurobiology, School of Basic Medical Sciences, Shanghai Medical University, China. smulib@public.sta.net.cn

Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica
|June 22, 1999
PubMed
Summary

Dihydroetorphine (DHE) tolerance involves changes in brain chemistry. Key amino acids like glutamic acid and GABA increase, while cAMP levels decrease in specific brain regions, indicating their role in opioid tolerance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Study on predictive indicators for early efficacy of cluster immunotherapy in patients with dust mite-induced asthma at a hospital in Liaoning Province from 2022 to 2024].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2026
Same author

[Analysis of the efficacy of halo-pelvic traction combined with posterior osteotomy in the treatment of severe rigid neurofibromatosis type 1 kyphoscoliosis].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2026
Same author

[A multicenter, prospective, observational study of cough variant asthma in China: clinical features, airway inflammation, and prognosis].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2025
Same author

[Advances in the application of anti-obesity medication for weight management in children and adolescents].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2025
Same author

[Research progress on pollen monitoring and pollen allergenicity of ragweed].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2024
Same author

[Effects of high-fat and low-carbohydrate diet combined with radiotherapy on tumor microenvironment of Lewis lung cancer bearing mice].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2024

Area of Science:

  • Neuroscience
  • Pharmacology
  • Biochemistry

Background:

  • Opioid tolerance is a significant clinical challenge, impacting treatment efficacy.
  • Understanding the neurochemical underpinnings of opioid tolerance is crucial for developing effective management strategies.

Purpose of the Study:

  • To investigate the neurochemical mechanisms underlying dihydroetorphine (DHE) tolerance in a mouse model.
  • To identify specific changes in amino acid and cyclic AMP (cAMP) concentrations associated with DHE tolerance.

Main Methods:

  • DHE tolerance was induced in mice through repeated subcutaneous injections with escalating doses over 8 days.
  • Amino acid concentrations were quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) with fluorescence detection.

Related Experiment Videos

  • Cyclic AMP (cAMP) levels were measured using radioimmunoassay.

    • Main Results:

    • Significant increases in basal brain concentrations of glutamic acid (Glu) (22.0%), aspartic acid (Asp) (36.7%), and gamma-aminobutyric acid (GABA) (77.8%) were observed in DHE-tolerant mice compared to controls.
    • Basal cAMP levels markedly decreased in the hypothalamus (64.6%) and striatum (65.6%) of tolerant mice.
    • No significant changes in glutamine (Gln) or cAMP levels in the cerebral cortex were detected.

    Conclusions:

    • The observed alterations in Glu, Asp, and GABA concentrations in the brain are implicated in the development of DHE tolerance.
    • The reduction in cAMP levels within the hypothalamus and striatum is also a key factor contributing to DHE tolerance.
    • These neurochemical changes provide insights into the complex mechanisms of opioid tolerance.