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Platelet-released ADP stabilizes PAF-induced rabbit platelet aggregation by stabilizing intracellular calcium
Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica
|June 22, 1999
Summary
Platelet-released adenosine diphosphate (ADP) stabilizes platelet aggregation induced by platelet-activating factor (PAF) in rabbits. This stabilization is achieved by maintaining elevated intracellular calcium levels.
Area of Science:
- Hematology
- Biochemistry
- Pharmacology
Background:
- Platelet aggregation plays a crucial role in hemostasis and thrombosis.
- Platelet-activating factor (PAF) is a potent inducer of platelet aggregation.
- The role of platelet-released adenosine diphosphate (ADP) in modulating PAF-induced aggregation requires further elucidation.
Purpose of the Study:
- To investigate the contribution of platelet-released ADP to the stabilization of PAF-induced rabbit platelet aggregation.
- To understand the mechanisms underlying ADP's role in aggregation stabilization.
Main Methods:
- Rabbit platelet aggregation was assessed using turbidimetry.
- ADP release was quantified using High-Performance Liquid Chromatography (HPLC).
- Intracellular calcium ([Ca2+]i) dynamics were measured with the fluorescent indicator Fura 2-AM.
Main Results:
- Platelet-activating factor (PAF) induced stable platelet aggregation.
- Subsequent addition of the ADP scavenger, apyrase, led to concentration-dependent deaggregation.
- PAF stimulated ADP release and a transient rise in [Ca2+]i, which was subsequently reduced by apyrase.
Conclusions:
- Platelet-released ADP stabilizes PAF-induced rabbit platelet aggregation.
- This stabilization is mediated by maintaining elevated intracellular calcium levels.