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Related Experiment Videos

A zinc-dependent Cl- current in neuronal somata.

T Tabata1, A T Ishida

  • 1Section of Neurobiology, Physiology, and Behavior, University of California, Davis, 95616-8519, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|June 23, 1999
PubMed
Summary

Cytoplasmic zinc (Zn2+) was found to regulate neuronal function by facilitating the downward regulation of a background chloride (Cl-) conductance via protein kinase C (PKC) in fish retinal ganglion cells.

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Area of Science:

  • Neuroscience
  • Cellular Electrophysiology
  • Trace Element Signaling

Background:

  • Extracellular Zn2+ is known to modulate ion channel activity.
  • The electrophysiological effects of intracellular Zn2+ at physiological concentrations are not well understood.
  • Investigating the role of cytoplasmic Zn2+ in neuronal function is crucial for understanding cellular excitability.

Purpose of the Study:

  • To investigate the effects of lower levels of cytoplasmic Zn2+ on neuronal somata.
  • To determine if cytoplasmic zinc influences ion channel conductances.
  • To elucidate the mechanisms by which cytoplasmic zinc modulates neuronal electrical properties.

Main Methods:

  • Whole-cell patch-clamp recordings were performed on fish retinal ganglion cell somata.

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  • Zn2+-selective chelators, Zn2+-preferring ionophores, and exogenous Zn2+ were utilized.
  • Electrophysiological parameters were measured to assess the impact of zinc modulation.

    • Main Results:

    • Cytoplasmic zinc was found to facilitate the downward regulation of a background Cl- conductance by endogenous protein kinase C (PKC).
    • This regulation was sustained by nanomolar levels of free Zn2+.
    • This represents a novel mechanism where zinc-dependent PKC controls neuronal conductance.

    Conclusions:

    • Cytoplasmic zinc plays a significant role in regulating neuronal excitability through modulation of background Cl- conductances.
    • The findings provide the first electrophysiological evidence for zinc-dependent PKC activity in neurons.
    • Background Cl- conductances are susceptible to modulation by intracellular zinc levels, impacting neuronal electrical properties.