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Updated: Oct 20, 2026
06:24
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Published on: April 21, 2019
[Comparative and quantitative analysis of proteins in the urine peritoneal fluid, separated by gel electrophoresis
E B Okon1, J Hranisavljevic, D Vucelic
1Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Russia. kaminsky@trik.iteb.serpukhov.su
Voprosy Meditsinskoi Khimii
|June 23, 1999
Abstract:
The method for the silver staining of proteins fractionated with SDS electrophoresis is described as a new approach for the complex analysis of biological fluids allowing to obtain the valuable diagnostic information and to determine quantitatively individual proteins in urine and peritoneal fluid, in particular in nephropathologies.
Related Concept Videos
01:22Two-dimensional Gel Electrophoresis
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
01:27SDS-PAGE
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...