[Laser spatial scanning in flow cytometry].

Area of Science:

• Biophysics
• Immunology
• Analytical Chemistry

Context:

• Flow laser cytometry is a powerful technique for analyzing biological particles based on light scattering.
• Immunological investigations and diagnostics require sensitive and accurate cell analysis methods.
• Existing flow cytometry systems face challenges in realization and utilization.

Purpose:

• To analyze the principles, applications, and challenges of flow laser cytometry.
• To describe a novel experimental flow cytometer model utilizing laser probing beam space scanning.
• To evaluate the sensitivity and applicability of the developed apparatus.

Summary:

• The study details flow laser cytometry principles, focusing on elastic light scattering.
• An original experimental model of a flow cytometer with laser probing beam space scanning is presented.
• The apparatus was validated using polystyrene latex spheres and biological samples, including red blood cells and bacteria.

Impact:

• The developed flow cytometer demonstrates sufficient sensitivity for detecting various biological objects.
• This research contributes to advancing flow cytometry techniques for immunological research and diagnostics.
• The findings support the potential of this novel apparatus in biological and medical applications.