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Antioxidant activity applying an improved ABTS radical cation decolorization assay.

R Re1, N Pellegrini, A Proteggente

  • 1International Antioxidant Research Centre, Guy's, King's and St Thomas' School of Biomedical Sciences, Kings College-Guy's Campus, London, UK.

Free Radical Biology & Medicine
|June 25, 1999
PubMed
Summary

This study introduces an improved antioxidant assay using 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation. The method enhances antioxidant screening for both hydrophilic and lipophilic compounds.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Antioxidant activity screening is crucial for understanding oxidative stress and disease.
  • Existing methods like the TEAC assay have limitations in scope and directness.
  • A need exists for a versatile and direct assay for diverse antioxidant compounds.

Purpose of the Study:

  • To develop and validate an improved decolorization assay for antioxidant activity screening.
  • To enhance the applicability of the ABTS radical cation assay for both lipophilic and hydrophilic antioxidants.
  • To refine the determination of antioxidant capacity by considering concentration and reaction time.

Main Methods:

  • Generation of the 2,2 -azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation via potassium persulfate oxidation.

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  • Utilizing a pre-formed ABTS radical cation for reduction by hydrogen-donating antioxidants.
  • Measuring inhibition of radical cation absorption, accounting for antioxidant concentration and reaction duration.

    • Main Results:

    • The assay successfully screens both lipophilic and hydrophilic antioxidants, including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants.
    • Direct generation of the ABTS radical cation simplifies the assay chemistry.
    • The decolorization assay allows for pre-formation of the radical, improving control over the reaction.

    Conclusions:

    • The improved ABTS decolorization assay offers a more direct and versatile method for antioxidant activity screening.
    • This assay is applicable to a wider range of antioxidant compounds and systems compared to the original TEAC assay.
    • The method provides a valuable tool for research in nutrition, pharmacology, and food science.