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Updated: Jul 29, 2026
12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Expression of SET is modulated as a function of cell proliferation
1Department of Biochemistry, College of Medicine and Research Institute for Genetic Engineering, Chungbuk National University, Cheongju, Korea.
Journal of Cellular Biochemistry
|June 25, 1999
Summary
The study investigated the biological role of SET, finding its expression decreases during cell differentiation. SET appears primarily involved in regulating cell proliferation, not differentiation, during embryonic development.
Area of Science:
- Developmental Biology
- Cell Biology
- Molecular Biology
Background:
- The protein SET's role in embryonic development is not fully understood.
- SET expression patterns and functions require further investigation.
Purpose of the Study:
- To explore the biological role of SET in cell proliferation and differentiation during embryonic development.
- To determine if SET expression levels correlate with cell cycle status.
Main Methods:
- Immunohistochemical staining of embryonic tissues to detect SET expression.
- Analysis of SET mRNA and protein levels in cultured cell lines (F9 teratocarcinoma, NIH 3T3, L6 myoblast, ECV304).
- Nuclear transport studies using enhanced green fluorescence protein-SET fusion proteins.
Main Results:
- SET expression was widespread in early embryonic development, localizing to specific tissues by embryonic day 19.
- SET protein was predominantly found in the nucleus.
- SET mRNA and protein levels decreased significantly upon differentiation of F9 teratocarcinoma cells.
- Serum starvation and cellular quiescence also led to reduced SET mRNA levels.
Conclusions:
- SET expression is dynamically regulated during embryonic development.
- SET appears to play a more significant role in regulating cell proliferation than cell differentiation.
- SET localization to the nucleus suggests a role in transcriptional regulation or cell cycle control.
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