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Updated: Aug 13, 2026

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
Published on: October 23, 2019
Chronic lymphocytic leukaemia presenting with central nervous system involvement
L Poplawska-Szczyglowska1, J Walewski, B Pienkowska-Grela
1Department of Lymphoproliferative Diseases, Centre of Oncology, Maria Sklodowska-Curie Memorial Institute, Warsaw, Poland.
Abstract:
A 68-year-old man presented with hemiparesis, lymphocytosis, and cerebral lesions on MRI. Flow cytometry of blood, bone marrow and cerebrospinal fluid showed B-CLL lymphocytes with bright CD20 expression, sIg, and absence of CD23 antigen. Fluorescence in situ hybridisation showed trisomy 12 in 50% of analysed peripheral mononuclear cells. The patient died 6 months after the diagnosis. Rapidly progressive and fatal course of the disease was consistent with known bad prognostic significance of CD20 bright expression and trisomy 12.
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