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Related Experiment Videos

Bacterial adhesion to functionalized polyurethanes.

R G Flemming1, R A Proctor, S L Cooper

  • 1Department of Chemical Engineering, University of Wisconsin, Madison 53706, USA.

Journal of Biomaterials Science. Polymer Edition
|June 29, 1999
PubMed
Summary
This summary is machine-generated.

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Bacterial adhesion to polyurethanes varies by surface chemistry. Phosphonated and zwitterionic polyurethanes show low Staphylococcus aureus adhesion, while quaternized amines show high adhesion, even with fibrinogen and high molecular weight kininogen present.

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Microbiology

Background:

  • Bacterial adhesion to medical implants is a major cause of infection.
  • Polyurethane surfaces are widely used in medical devices.
  • Understanding protein interactions with polyurethanes is crucial for developing infection-resistant materials.

Purpose of the Study:

  • To investigate the effect of fibrinogen and high molecular weight kininogen on bacterial adhesion to various functionalized polyurethanes.
  • To compare bacterial adhesion on different polyurethane chemistries.
  • To determine the influence of protein pre-coating on bacterial colonization.

Main Methods:

  • Coating glass slides with Pellethane, sulfonated Pellethane, phosphonated Pellethane, zwitterionic phosphonated polyurethane, and quaternized amine polyurethanes.

Related Experiment Videos

  • Exposing polymer-coated surfaces to radiolabeled Staphylococcus aureus.
  • Pre-treating polymers with varying concentrations of canine fibrinogen and/or two-chain high molecular weight kininogen (TCHMWK) before bacterial exposure.

    • Main Results:

    • Phosphonated Pellethane and zwitterionic phosphonated polyurethane exhibited the lowest bacterial adhesion, while methyl quaternized polyurethanes showed the highest.
    • Fibrinogen significantly increased bacterial adhesion on most polyurethanes, except for quaternized amine polyurethanes.
    • High molecular weight kininogen reduced fibrinogen-mediated bacterial adhesion on most surfaces, but not on quaternized amine polyurethanes.

    Conclusions:

    • Polyurethane surface chemistry significantly impacts bacterial adhesion.
    • Quaternized amine polyurethanes demonstrate inherent resistance to protein-mediated modulation of bacterial adhesion.
    • Developing biomaterials with specific surface properties can mitigate bacterial colonization on medical implants.