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Granulocyte colony-stimulating factor (G-CSF) transiently suppresses mitogen-stimulated T-cell proliferative response
E Reyes1, I García-Castro, F Esquivel
1Department of Medicine Principe de Asturias University Hospital, Alcalá University, Carretera Madrid-Barcelona, Alcalá de Henares, Madrid, Spain.
British Journal of Cancer
|July 2, 1999
Summary
Granulocyte colony-stimulating factor (G-CSF) treatment in breast cancer patients increased white blood cell counts but reduced peripheral blood mononuclear cell (PBMC) proliferation. This effect on immune cell function normalized after G-CSF therapy concluded.
Area of Science:
- Immunology
- Hematology
- Oncology
Background:
- Granulocyte colony-stimulating factor (G-CSF) is a cytokine crucial for neutrophil development and immune modulation.
- G-CSF is utilized in cancer therapy to mobilize peripheral blood stem cells.
Purpose of the Study:
- To investigate the impact of G-CSF on ex vivo peripheral blood mononuclear cell (PBMC) functions in breast cancer patients.
- To assess changes in immune cell populations and function during G-CSF administration for stem cell mobilization.
Main Methods:
- Peripheral blood samples were collected from breast cancer patients receiving r-metHuG-CSF and from healthy controls.
- Flow cytometry was used to analyze changes in immune cell subsets (CD19+, CD14+, CD45RA+, CD45RO+).
- PBMC proliferative responses to mitogenic stimulation were measured, alongside cytokine production patterns.
Main Results:
- G-CSF treatment significantly increased peripheral blood leukocyte, neutrophil, lymphocyte, and monocyte counts.
- Modulations were observed in the percentages of specific PBMC subsets, including B cells, monocytes, and T cell subsets.
- A significant reduction in PBMC proliferative capacity was noted during G-CSF treatment, which resolved 14 days post-therapy.
- No significant alterations in cytokine production patterns were associated with G-CSF administration.
Conclusions:
- G-CSF administration influences peripheral blood cell counts and PBMC function in breast cancer patients undergoing stem cell mobilization.
- The observed immunoregulatory effect, particularly the reduction in PBMC proliferation, may be indirect, as G-CSF receptors are absent on T lymphocytes.
- Further research is needed to elucidate the precise mechanisms and potential clinical implications of G-CSF-induced immunomodulation.