Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temperature-dependent conformational changes in a voltage-gated potassium channel.

J K Tiwari1, S K Sikdar

  • 1Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.

European Biophysics Journal : EBJ
|July 8, 1999
PubMed
Summary

This study investigated temperature's effect on voltage-gated potassium (K+) channel gating in alpha T3-1 cells. Increased temperature facilitates voltage sensor movement, easing channel opening and closing dynamics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding factors associated with continuation of intrauterine device use in Gujarat and Rajasthan, India: a cross-sectional household study.

Sexual and reproductive health matters·2021
Same author

L-pGlu-(2-propyl)-L-His-L-ProNH₂ attenuates 4-aminopyridine-induced epileptiform activity and sodium current: a possible action of new thyrotropin-releasing hormone analog for its anticonvulsant potential.

Neuroscience·2011
Same author

Inhibition of human two-pore domain K+ channel TREK1 by local anesthetic lidocaine: negative cooperativity and half-of-sites saturation kinetics.

Molecular pharmacology·2009
Same author

Glutamate transporter blockade affects Ca(2+) responses in astrocytes.

Neuroscience·2007
Same author

Time-dependent molecular memory in single voltage-gated sodium channel.

The Journal of membrane biology·2007
Same author

Characterization of contryphans from Conus loroisii and Conus amadis that target calcium channels.

Peptides·2006

Area of Science:

  • Biophysics
  • Cellular Physiology
  • Ion Channel Function

Background:

  • Voltage-gated potassium (K+) channels are crucial for cellular electrical excitability.
  • Conformational changes in K+ channels are essential for their gating mechanism.
  • Understanding the energetic landscape of channel gating provides insights into cellular signaling.

Purpose of the Study:

  • To investigate the energetic changes associated with conformational changes during the gating of non-inactivating voltage-gated K+ channels.
  • To utilize temperature as a biophysical tool to probe these energy changes.
  • To characterize the temperature dependence of K+ channel gating kinetics and thermodynamics.

Main Methods:

  • Utilized electrophysiology to measure K+ channel currents in alpha T3-1 cells at varying temperatures (15, 25, 35°C).

Related Experiment Videos

  • Analyzed current activation time courses using single exponential functions.
  • Applied Arrhenius plots to determine activation energies of rate constants.
  • Estimated Gibb's free energy changes using two distinct approaches at different membrane potentials.

    • Main Results:

    • K+ channel current activation followed single exponential kinetics across tested temperatures.
    • Q10 values ranged from 1.5 to 1.9, consistent with activation energy calculations.
    • Gibb's free energy changes for channel opening/closing were similar across estimation methods.
    • Depolarization-dependent Gibb's free energy changes exhibited a quadratic relationship, more pronounced at lower temperatures.

    Conclusions:

    • Temperature influences the conformational dynamics of voltage-gated K+ channels.
    • Increased temperature favors the movement of voltage-sensing segments, reducing constraints within the channel molecule.
    • These findings contribute to a deeper understanding of the biophysics underlying ion channel function and regulation.