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The structure and organization of two cysteine endopeptidase genes from rice
H Kato1, A Shintani, T Minamikawa
1Department of Biological Sciences, Tokyo Metropolitan University, Japan.
Abstract:
REP-1 is a major cysteine endopeptidase that digests seed storage glutelin of rice. A cDNA clone (pRP60) for REP-1 and a cDNA clone (pRP80) for a related enzyme were previously isolated. The expression of both mRNAs is regulated by gibberellin. In this study, we revealed the structure and organization of Rep1 and RepA, genes corresponding to pRP60 and pRP80 mRNAs, respectively. Rep1 has no introns whereas RepA consists of five exons and four introns, and both genes exist as one copy gene in the rice genome. The gibberellin-responsive elements conserved in cereal alpha-amylase genes are not included in the 5'-upstream region of Rep1 or RepA. A molecular phylogenetic tree of plant cysteine endopeptidases was constructed, and their relationship was discussed.