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Polymorphonuclear cell apoptosis in exudates generated by polymers.

T Fabre1, F Belloc, B Dupuy

  • 1INSERM U-443, Université de Bordeaux II, 146 rue Leo-Saignat, 33076 Bordeaux Cedex, France.

Journal of Biomedical Materials Research
|July 9, 1999
PubMed
Summary
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Biomaterials induce higher rates of polymorphonuclear (PMN) cell apoptosis and necrosis in exudates compared to blood. Apoptosis is a key process for PMN cell removal and inflammation control during early inflammatory responses.

Area of Science:

  • Biomaterials Science
  • Immunology
  • Cell Biology

Background:

  • Polymorphonuclear (PMN) cells play a crucial role in the inflammatory response.
  • Understanding PMN cell death pathways, apoptosis and necrosis, is vital for evaluating biomaterial interactions.
  • Biomaterial-induced inflammation can lead to altered PMN cell kinetics.

Purpose of the Study:

  • To quantify and compare apoptosis and necrosis of PMN cells in biomaterial-induced exudates versus peripheral blood.
  • To investigate the temporal dynamics of PMN cell apoptosis and necrosis following biomaterial implantation.
  • To explore the influence of different biomaterials and serum on PMN cell death.

Main Methods:

  • Flow cytometry utilized for quantifying apoptotic and necrotic PMN cells.

Related Experiment Videos

  • Hoechst 33342 and propidium iodide staining employed for identifying apoptotic and permeabilized PMN cells, respectively.
  • Subcutaneous implantation of biomaterial-filled tubes in rats to generate exudates over a 7-day period.

    • Main Results:

    • PMN cell apoptosis and necrosis were significantly higher in biomaterial exudates than in rat peripheral blood.
    • Apoptosis peaked at day 2 (27% +/- 6%), while permeabilized cells increased over time, exceeding apoptotic cells by days 4 and 8.
    • Serum (10%) inhibited PMN cell apoptosis and necrosis, and different biomaterials (polyurethane vs. PVC/TMC-ECL) showed varying apoptosis rates.

    Conclusions:

    • Apoptosis is the primary programmed cell death pathway for PMN cells in early-stage biomaterial-induced inflammation.
    • Apoptosis serves as an organized mechanism for PMN cell removal, thereby limiting inflammation.
    • Necrosis and apoptosis represent distinct pathways for PMN cell death in the exudate environment.