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Common and HIV-related diffuse large B-cell lymphomas differ in their immunoglobulin gene mutation pattern
H J Delecluse1, M Hummel, T Marafioti
1Consultation and Reference Centre for Lymph Node Pathology and Hematopathology, Institute of Pathology, Klinikum Benjamin Franklin, Free Univerisity of Berlin, Germany.
The Journal of Pathology
|July 9, 1999
Summary
Diffuse large B-cell lymphomas (DLBCL) in HIV patients are distinct from those in the general population. Many HIV-DLBCL cases are linked to Epstein-Barr virus (EBV) and originate from early B-cells.
Area of Science:
- Immunology
- Oncology
- Virology
Background:
- Patients with HIV are highly susceptible to diffuse large B-cell lymphomas (DLBCL).
- The origin of HIV-related DLBCL remains unclear, with debate on whether they are Epstein-Barr virus (EBV)-driven or similar to general population DLBCL.
Purpose of the Study:
- To investigate the characteristics of immunoglobulin heavy chain (IgH) genes and EBV association in HIV-related DLBCL.
- To differentiate HIV-DLBCL from DLBCL in the general population.
Main Methods:
- Analysis of clonally rearranged and somatically mutated immunoglobulin heavy chain (IgH) genes in 12 HIV-DLBCL samples.
- Detection of Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) and EBV-encoded nuclear antigen 2 (EBNA-2) expression.
Main Results:
- Eleven of 12 samples showed monoclonal IgH gene rearrangements, with moderate somatic mutations.
- Ten of 12 samples expressed EBERs, and six also expressed EBNA-2.
- HIV-DLBCL exhibited fewer somatic IgH mutations and a higher association with EBV compared to general population DLBCL.
Conclusions:
- HIV-related DLBCL is a distinct entity, often originating from EBV-driven lymphoproliferation.
- Approximately half of HIV-DLBCL cases appear to arise from pre-germinal center B-cells.