Related Experiment Videos
[Inhibition of nuclear histone proteolysis by nucleotides]
V I Tiulenev1, L A Konoplich, O A Rudenko
1T. Shevchenco Kyiv State University, Ukraine.
Ukrainskii Biokhimicheskii Zhurnal (1978)
|July 14, 1999
Summary
Nucleotides like ATP protect histones from proteolysis, with H4 histone showing resistance. This indicates nucleotides are crucial for maintaining chromatin structure and histone integrity.
Area of Science:
- Molecular Biology
- Biochemistry
Context:
- Histones are fundamental proteins that package DNA into chromatin.
- Proteolysis can alter chromatin structure and function.
- Nucleotides play diverse roles within the cell nucleus.
Purpose:
- To investigate the impact of various nucleotides on histone proteolysis in isolated nuclei.
- To identify specific histones affected by proteolysis and the protective roles of nucleotides.
Summary:
- Histone H1 was initially removed during proteolysis, followed by H3 and H2B digestion, while H4 remained intact.
- Phenylmethylsulfonyl fluoride (PMSF) and adenosine triphosphate (ATP) inhibited H1 cleavage.
- Adenosine triphosphate (ATP), cytidine triphosphate (CTP), adenosine diphosphate (ADP), nicotinamide adenine dinucleotide (NAD+), adenosine monophosphate (AMP), and nicotinamide adenine dinucleotide (NADH) inhibited partial cleavage of core histones H3 and H2B.
- ATP, CTP, AMP, and NADH prevented complete H2B digestion.
- ATP and CTP inhibited H3 digestion.
- ATP, CTP, ADP, and NAD+ inhibited H2A cleavage during prolonged incubation.
- Histone H4 demonstrated resistance to cleavage in the absence of nucleotides.
Impact:
- The findings highlight the significant role of ATP and other nucleotides in preserving histone structure.
- Nucleotides are essential for maintaining the overall integrity of chromatin.
- This research provides insights into the post-translational modifications and stability of chromatin components.