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Related Experiment Videos

The Feulgen reaction 75 years on.

P Chieco1, M Derenzini

  • 1Istituto di Oncologia F. Addarii, Policlinico S. Orsola, Bologna, Italy.

Histochemistry and Cell Biology
|July 14, 1999
PubMed
Summary
This summary is machine-generated.

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The Feulgen reaction stains DNA in situ using Schiff reagents after HCl hydrolysis. While DNA quantification is its main use, new electron microscopy applications reveal DNA

Area of Science:

  • Biochemistry
  • Cell Biology
  • Histochemistry

Background:

  • The Feulgen reaction, developed 75 years ago, is a widely used cytohistochemical method.
  • It specifically stains DNA in situ by reacting Schiff reagents with aldehyde groups generated by HCl hydrolysis.
  • Staining intensity correlates with DNA concentration.

Purpose of the Study:

  • To review the applications and principles of the Feulgen reaction.
  • To highlight its current use in DNA quantification for tumor pathology.
  • To explore emerging applications in electron microscopy for DNA structural studies.

Main Methods:

  • Utilizes the Feulgen reaction involving hydrochloric acid hydrolysis and Schiff or Schiff-like reagents.
  • Employs image cytometry for DNA quantification in cell nuclei.

Related Experiment Videos

  • Applies Feulgen principles to electron microscopy techniques.

    • Main Results:

    • The Feulgen reaction enables specific DNA staining, with intensity proportional to DNA concentration.
    • Current primary application is DNA quantification in cell nuclei for ploidy evaluation in tumor pathology.
    • Recent adaptations for electron microscopy allow detailed study of in situ DNA structural organization.

    Conclusions:

    • The Feulgen reaction remains a valuable tool in biology and medicine.
    • Its application has shifted from morphological studies to quantitative DNA analysis and advanced structural investigations.
    • New electron microscopy methods extend the utility of the Feulgen reaction for studying DNA organization.