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Updated: Oct 19, 2026

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
Published on: May 16, 2017
Poly(ADP-ribose) polymerase is found in both the nucleus and cytoplasm of human CNS neurons
M R Cookson1, P G Ince, P A Usher
1Department of Neurology, University of Newcastle upon Tyne, 1 Framlington Place, Newcastle upon Tyne, NE2 4HH, UK. m.r.cookson@ncl.ac.uk
Abstract:
There is evidence that inhibitors of poly(ADP-ribose) polymerase (PARP) may be therapeutically useful in neurodegenerative diseases. Using immunocytochemistry, we have investigated the distribution of PARP in the human CNS. Some neuronal groups showed cytoplasmic staining in addition to the expected staining of nuclei. Considerable variation between different neuronal groups was noted: motor neurons in the spinal cord showed greatest cytoplasmic staining, whereas staining was virtually absent in other neurons, notably in the hippocampus. These results indicate that PARP can be associated with sub-cellular components other than the nucleus, and may indicate additional roles for this enzyme.
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