Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Dec 7, 2025

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

Published on: September 23, 2014

Tissue microarray (TMA) use in post mortem neuropathology.

L A Wilson¹, L Heraty², B A Ashford³

¹Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Midlothian, EH25 9RG, UK.

Journal of Neuroscience Methods

|October 2, 2020

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ophthalmic Features in Stroke Patients: A Systematic Review of Imaging Studies.

Current eye research·2026

Same author

How does urban morphology impact cities air quality? A modelling study.

The Science of the total environment·2025

Same author

Correction: Upregulated c-myc expression in multiple myeloma by internal ribosome entry results from increased interactions with and expression of PTB-1 and YB-1.

Oncogene·2024

Same author

Squirrelpox in a red squirrel in Fife.

The Veterinary record·2024

Same author

Facing the challenges of air quality and health in a future climate: The Aveiro Region case study.

The Science of the total environment·2023

Same author

Source apportionment of air pollution in European urban areas: Lessons from the ClairCity project.

Journal of environmental management·2022

Same journal

Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

Journal of neuroscience methods·2026

Same journal

Assessment metrics for pain control in rats: A methodological commentary.

Journal of neuroscience methods·2026

Same journal

Infant EEG preprocessing pipelines: A capability framework and current gaps in practice.

Journal of neuroscience methods·2026

Same journal

Methods for measuring neural activity during voluntary wheel running.

Journal of neuroscience methods·2026

Same journal

Serotype-dependent differences in AAV cellular transduction rates in the hypothalamus of Arctic ground squirrels.

Journal of neuroscience methods·2026

Same journal

Rapid generation of human sensory neurons from iPSC for modeling of peripheral neuropathies.

Journal of neuroscience methods·2026

See all related articles

This summary is machine-generated.

Tissue microarrays (TMAs) enable efficient neuropathology research by using less tissue, time, and reagents. Studies show TMAs provide comparable results to whole sections for assessing microglial pathology and TDP-43 proteinopathy.

Area of Science:

Neuropathology
Biomedical research methodology
Tissue analysis

Background:

Tissue microarrays (TMAs) offer potential for more efficient use of tissue, reagents, and time in neuropathology.
TMAs have been underutilized in post-mortem neuropathology research.

Purpose of the Study:

To investigate the relationship between data from TMA cores and whole tissue sections.
To develop and validate a protocol for TMA construction suitable for neuropathology.

Main Methods:

Quantitative assessment of microglial pathology in white matter lesions and motor neuron disease using 'virtual' TMA cores.
Qualitative assessment of TDP-43 inclusion status in motor neuron disease cases.
Development of a protocol for TMA construction and immunohistochemistry.

Keywords:

Formalin fixed paraffin embedded Immunohistochemistry Neurodegeneration Neuropathology Post mortem Tissue microarray

More Related Videos

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis

Published on: July 19, 2019

Related Experiment Videos

Last Updated: Dec 7, 2025

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

Published on: September 23, 2014

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis

Published on: July 19, 2019

Main Results:

One 1.75 mm core showed good concordance with whole sections for microglial volume density.
Two cores were required for more sophisticated microglial measures.
Single cores achieved 100% sensitivity and specificity for TDP-43 in grey matter and high sensitivity/specificity in white matter.
Developed TMA construction method is suitable for manual and automated immunohistochemistry with minimal tissue loss.

Conclusions:

TMAs can effectively assess both quantitative (microglial pathology) and qualitative (TDP-43 proteinopathy) features using significantly reduced tissue, time, and reagents.
TMAs provide comparable results to whole sections and can be constructed and stained with relative ease.
TMAs hold promise for improving data acquisition efficiency in neuropathology research.