Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eukaryotic conditional expression system.

T Sourisseau1, Y Le Dréan, G Salbert

  • 1Université de Rennes 1, France.

Biotechniques
|July 17, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unveiling how mitotic spindle orientation in 3D human colon organoids affects matrix displacements through a 4D study using DVC.

Scientific reports·2025
Same author

Culture yield of 1st and 2nd biopsy in bacterial non-mycobacterial spondylodiscitis in a university hospital.

Infectious diseases now·2025
Same author

Competing magnetic phases and fluctuation-driven scalar spin chirality in the kagome metal YMn<sub>6</sub>Sn<sub>6</sub>.

Science advances·2020
Same author

Miller-Fisher syndrome after COVID-19: neurochemical markers as an early sign of nervous system involvement.

European journal of neurology·2020
Same author

COVID-19 Neurologic Complication with CNS Vasculitis-Like Pattern.

AJNR. American journal of neuroradiology·2020
Same author

Effectiveness of antibacterial prophylaxis in children with acute leukemia: A report from a single institution over a 20-year period.

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2018

This study introduces a novel conditional gene expression system that combines transcriptional induction and de-repression. This innovative system eliminates basal expression, offering precise control over gene activity using a ligand-switchable transcription factor.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Biotechnology

Background:

  • Conditional gene expression systems are crucial for controlling gene activity.
  • Existing systems primarily rely on either transcriptional induction or de-repression.
  • A need exists for systems with enhanced control and absence of basal expression.

Purpose of the Study:

  • To develop a novel conditional gene expression system combining induction and de-repression mechanisms.
  • To achieve precise control over gene transcription with no basal expression.
  • To demonstrate the efficacy of a ligand-switchable transcription factor system.

Main Methods:

  • Construction of a chimeric protein (GalER) fusing the yeast Gal4 DNA-binding domain with the estradiol receptor.

Related Experiment Videos

  • Utilizing an optimized Gal4-responsive promoter.
  • Assessing system efficacy via chloramphenicol acetyl transferase (CAT) transient expression assays.
  • Evaluating gene expression in situ using a Gal4-responsive beta-galactosidase gene.

    • Main Results:

    • The developed system effectively combines transcriptional induction and de-repression.
    • The chimeric protein (GalER) functions as a repressor in the presence of 4OH-tamoxifen and switches to activation upon ligand change.
    • Complete absence of basal expression was observed prior to induction.
    • Successful validation in transient and in situ expression assays.

    Conclusions:

    • A novel, highly efficient conditional gene expression system has been established.
    • The system offers precise, ligand-dependent control over gene transcription.
    • This method provides a significant advantage by eliminating basal expression, enhancing experimental accuracy.