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Synthesis of a monocharged peptide nucleic acid (PNA) analog and its recognition as substrate by DNA polymerases
M J Lutz1, D W Will, G Breipohl
1Swiss Federal Institute of Technology, Department of Chemistry, Zürich, Switzerland.
Nucleosides & Nucleotides
|July 17, 1999
Abstract:
The preparation of a novel phosphoramidite monomer based on thyminyl acetic acid coupled to the secondary nitrogen of 2-(2-amino-ethylamino)ethanol is described. This monomer can be used to attach a deoxynucleotide to the carboxy terminus of a PNA oligomer by solid-phase synthesis. The resulting PNA primer is recognized as a substrate by various DNA polymerases.