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Updated: Aug 15, 2026
Culturing Microglia from the Neonatal and Adult Central Nervous System
Published on: August 9, 2013
Inducible nitric oxide synthase expression in cultures enriched for mature oligodendrocytes is due to microglia
Abstract:
Hewett JA, Hewett SJ, Winkler S, Pfeiffer SE. 1999. Inducible nitric oxide synthase expression in cultures enriched for mature oligodendrocytes is due to microglia. J Neurosci Res 56:189-198. In the article referenced above, the LPS concentration employed in all studies was 1 &mgr;g/ml, not 1 mg/ml as published. This correction appears: in the Materials and Methods section on page 190, column 1, line 47; in the Results section on page 191, column 2, legend to Figure 1, line 3; on page 192, column 2, legend to Figure 2, line 3; on page 194, column 2, legend to Figure 5, line 4; on page 195, column 1, legend to Figure 6, line 3; and in the Discussion section on page 196, column 1, line 9. The publisher regrets this error.
Insights
Microglia, not mature oligodendrocytes, express inducible nitric oxide synthase in cell cultures. This correction clarifies that lipopolysaccharide (LPS) concentration was 1 µg/ml, not 1 mg/ml, in the original study.
Area of Science:
- Neuroscience
- Immunology
Background:
- Mature oligodendrocytes are crucial for myelin sheath maintenance in the central nervous system.
- Microglia are the resident immune cells of the central nervous system and play a role in neuroinflammation.
- Inducible nitric oxide synthase (iNOS) is an enzyme involved in immune responses and can produce nitric oxide, a signaling molecule.
Purpose of the Study:
- To investigate the cellular source of inducible nitric oxide synthase (iNOS) expression in cultures enriched for mature oligodendrocytes.
- To clarify the concentration of lipopolysaccharide (LPS) used in the original study.
Main Methods:
- Primary mixed glial-oligodendrocyte cultures were prepared.
- Cultures were treated with lipopolysaccharide (LPS).
- Expression of iNOS was assessed.
Main Results:
- Inducible nitric oxide synthase (iNOS) expression was primarily observed in microglia, not in mature oligodendrocytes.
- A correction was issued regarding the LPS concentration used, clarifying it was 1 µg/ml, not 1 mg/ml.
Conclusions:
- The findings indicate that microglia are the main source of iNOS in these experimental conditions.
- This correction is essential for the accurate interpretation of the study's results concerning neuroinflammation and oligodendrocyte responses.
