Related Experiment Videos
Is another mannose receptor-like lectin present in retinal pigment epithelium?
1Department of Anatomical Sciences & Neurobiology, University of Louisville School of Medicine, KY 40202, USA.
Purpose:
This study was designed to investigate the presence of the secretory phospholipase A2 receptor in RPE, a protein closely related to the phagocytic mannose receptor.
Methods:
Proteins from cultured rat, pig, and human RPE were separated by SDS-PAGE and immunoblotted with a polyclonal guinea pig sPLA2 receptor antibody. RT-PCR was performed on rat and pig RPE samples using primers designed from published rat pancreatic sPLA2 receptor sequences.
Results:
The sPLA2 receptor protein was not detected in rat, pig, or human RPE by immunoblots. Additionally, message for this receptor was not detected in rat or pig RPE.
Conclusions:
With these techniques, these data demonstrate that the sPLA2 receptor is undetectable in the RPE.