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Related Experiment Videos

Actin motion on microlithographically functionalized myosin surfaces and tracks.

D V Nicolau1, H Suzuki, S Mashiko

  • 1Osaka National Research Institute, Osaka 563, Japan. dan.nicolau@riotinto.com.au

Biophysical Journal
|July 29, 1999
PubMed
Summary

Electron beam patterning of polymer resists changes surface hydrophobicity, enabling selective heavy meromyosin attachment. This controlled surface modification influences actin filament motion, paving the way for molecular motor arrays.

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Area of Science:

  • Materials Science
  • Biophysics
  • Nanotechnology

Background:

  • Polymer resists like poly[(tert-butyl-methacrylate)-co-(methyl methacrylate)] are crucial in semiconductor microlithography.
  • Surface properties, such as hydrophobicity, can be precisely altered using techniques like electron beam patterning.
  • Understanding molecular motor behavior on patterned surfaces is key for developing novel nanodevices.

Purpose of the Study:

  • To investigate how electron beam patterning of a common polymer resist affects surface hydrophobicity.
  • To examine the selective attachment of heavy meromyosin to these patterned surfaces.
  • To characterize the motion of actin filaments on surfaces with varying myosin densities and explore potential applications in molecular motor arrays.

Main Methods:

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  • High-resolution electron beam (e-beam) patterning was used to modify the surface of poly[(tert-butyl-methacrylate)-co-(methyl methacrylate)].
  • Surface hydrophobicity changes were measured after e-beam exposure.
  • Heavy meromyosin was selectively attached to hydrophobic regions, creating myosin-rich and myosin-poor areas.
  • Actin filament movement was statistically analyzed for velocity, acceleration, and angle on these surfaces.

    • Main Results:

    • E-beam patterning induced significant changes in surface hydrophobicity.
    • Heavy meromyosin selectively attached to unexposed, hydrophobic regions of the resist.
    • Actin filaments exhibited smoother motion on myosin-rich surfaces compared to uneven motion on myosin-poor surfaces.
    • A mild slowing effect on actin filament motion was observed with excess myosin, and the myosin-rich/myosin-poor boundary showed an alignment-enforcement effect.

    Conclusions:

    • Electron beam patterning offers a method to create surfaces with controlled hydrophobicity for selective biomolecule immobilization.
    • The study demonstrates the influence of surface properties and molecular motor density on actin filament dynamics.
    • These findings support the feasibility of designing and constructing ordered molecular motor arrays for potential applications in nanotechnology and biophysics.